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The Conservation Action Network (C.A.N.)


In 1998, the Memphis Zoo created the Conservation Action Network (C.A.N.), the grants division for supporting local, national and international conservation projects for endangered species and their habitats. We firmly believe that every zoo C.A.N. make a difference for the continued survival of the animals and plants we hold in public trust. Since its inception, the zoo has awarded more than $300,000 to zoo staff and our research associates for conducting projects in the U.S., Brazil, China, Latin America, Caribbean, Russia and Africa. The application process is by invite only and has allowed the Memphis Zoo to focus on its core mission for select species and habitats while supporting the professional development and experience of our employees and partner institutions. Each spring the zoo awards $25,000-$40,000 in grant funds to projects that are peer-reviewed by our external scientific advisory committee (SAC). Once these evaluations are completed the CAN board members review the comments and make funding decisions in late April.
The Memphis Zoo raises conservation dollars through several venues including operational support, wishing well fountains throughout the zoo, Individual donors, conservation fund check-off on zoo membership, donation boxes, and our annual fundraiser Wild World of Wines and Beer. The success of our projects has been the result of a grass-roots effort and we thank the Memphis community for all their support throughout the years. Your continued support of C.A.N. is greatly appreciated – if you would like to make a donation to C.A.N. and help save our remaining wildlife please click here to find out how. We would like to Thank our Scientific Advisory Committee and C.A.N. board for all their time and resources they devote to making the Memphis Zoo a world-renowned conservation organization.
Scientific Advisory Committee: Conservation Board:
Dr. Judy Cole – University of Memphis
Dr. Karyl Buddington – University of Memphis
Dr. Rosanna Capelatto – Rhodes College
Dr. Mary Ogilvie – Christian Brothers University
Dr. Ashli Brown – Mississippi State University





  Dr. Joe DeWane – committee chair   
Gene Holcomb
Dorothy Kirsch
Steve Priddy            
Irvin Serenco
Brooke Sparks
John Stokes, Jr.
Diana Hull
Steven Underwood
Roger Knox
 
Conservation Action Network Projects Awarded for 2009-2010

1. Development of reproductive monitoring techniques for captive management of endangered Amur and snow leopards


The Amur leopard is a critically endangered subspecies of the leopard and is in immediate danger of extinction.  A reintroduction plan using offspring from captive animals has been proposed to restore the Amur leopards’ range in the Russian Far East and supplement the existing population.  Similarly, the population of the endangered snow leopard is declining and captive breeding programs are not optimized to their full potential.  Little is known about the reproductive biology of either Amur or snow leopards.  Therefore, the goal of this study is to use a non-invasive technique to characterize and monitor reproductive steroid hormones in these leopards to increase the success of captive breeding programs.  The specific objectives are to 1) characterize seasonal fecal testosterone levels in pubertal and adult male leopards; and to 2) characterize seasonal fecal estrogens and progestins throughout the estrous cycle, ovulation, and pregnancy in pubertal and adult female leopards.  Fecal steroid hormone concentrations will be analyzed via enzyme immunoassay (EIA) in both breeding pairs and singletons.  Fecal samples will be collected every other day for 60 days out of each season.  Results from this study will help define differences among steroid profiles of pubertal and adult animals, determine type of ovulation, more closely define seasonal changes in reproductive activity, and assist with pregnancy diagnosis.  Knowledge of basic reproductive factors will enhance the ability of captive breeding programs to increase pregnancy and birth rates among Amur and snow leopards, and will aid the in preparation of a reintroduction program for Amur leopards.

2. Habitat characteristics of Chinese giant salamanders in nature and captivity


The Chinese giant salamander (Andrias davidianus) is classified as critically endangered by the International Union for Conservation of Nature and Natural Resources, a Class II state major protected species in China, and is included in Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Giant salamanders are in peril because of habitat loss, their relatively slow growth to sexual maturity (6 years), and poaching for human consumption and medicinal use. Chinese giant salamander breeding centers and nature reserves have existed for decades, yet captive breeding has remained difficult and nature reserves are failing to protect remaining populations. To augment efforts of breeding centers, we propose a bipartite in-situ (in nature) and ex-situ (in captivity) study of the habitat requirements of Chinese giant salamanders in the Foping National Nature Reserve, Shaanxi Province, China. We will study salamanders in situ to identify local populations and specific microhabitat use and to quantify vital habitat characteristics including stream morphology, chemistry, and temperature, and adjacent vegetation community and canopy cover. We will study simulated giant salamander habitat ex situ at existing breeding centers to quantify the same habitat characteristics. We will analyze data in the laboratory at the Memphis Zoo to identify differences between in situ and ex situ habitats and define critical habitat components. This information will then be used in collaboration with breeding center managers to develop a strategy to incorporate key habitat features in ex situ breeding centers.

3. Evaluation of Human Serological Tests and Seroprevalence of Baylisascaris procyonis in Non-Human Primates


Infection with Baylisascaris procyonis, the raccoon roundworm, has recently been recognized as an emerging helminthic disease of both animals and humans.  The risk of infection is thought to be much greater than currently recognized, and the CDC has even considered the disease a possible agent of bioterrorism.  B. procyonis has a widespread geographic distribution with infection rates in raccoons as high as 100% and eggs that are extremely resistant, persisting in the environment for years.  Larvae have been found in more than 120 species of wild and domestic mammals and birds, and have caused fatal outbreaks on farms and in zoos where raccoon colonies cannot be controlled.  When ingested by a potential intermediate host, the larvae undergo aggressive somatic migration leading to visceral, ocular, and neural larva migrans (NLM) – all potentially severe, if not fatal, and of which there is currently no effective treatment.  NLM is particularly devastating even with only 5% of the larvae invading the brain.  Primates appear to be very susceptible to NLM, and there have been at least 4 suspected cases at the Memphis Zoo in the last 5 years.  Antemortem diagnosis is difficult, often limited to linking neurological signs with a positive serologic test.  However, serology tests for non-human primates have not been validated.  This study hopes to determine the prevalence and incidence of B. procyonis in zoo primates and help determine if current reagents and cutoff values for serologic tests in humans could also apply to non-human primates. If they do, then current testing could be used as a noninvasive diagnostic tool for non-human primates.

4. Mariana Avifauna Conservation Program Phase V Translocation of Threatened Species


The primary goal of Phase V of the Mariana Avifauna Conservation Program (MAC) will be the translocation of the golden white-eye (Cleptornis marcheii).  The golden white-eye is only found on the islands of Saipan and Aguigan. The introduced brown treesnake (bts) poses a threat to all species found on Saipan.  The biologists for the Commonwealth of the Northern Mariana Islands (CNMI) Division of Fish & Wildlife (DFW) working with biologists from the United States Fish & Wildlife Service (USFW) are evaluating factors that will influence the precise details of the translocation. The destination site chosen by CNMI biologists is Sarigan, a snake free, human free managed reserve island. The number of birds translocated will likely be 50 based on data from other successful translocations. I have included the bio-profiles for golden white-eyes from the Comprehensive Wildlife Conservation Strategy for the Commonwealth of the Northern Mariana Islands. Captive husbandry information has been added by Anne Tieber, species manager from the St. Louis Zoo. Part of the strategy for both species is translocation and captive management.   The secondary goal will be to collect additional specimens of species designated for captive breeding programs. How many and of which species will be based on an evaluation of existing populations currently being managed. It is probable that additional golden white-eyes, Tinian monarchs (Monarcha takatsukasae) and rufous fantails (Rhipidura rufifrons) will be collected.

5. A Behavioral Observation Study on the Group Dynamics of Subadult/Adult Captive Francois’ Langurs (Trachypithecus francoisi): A Coming of Age Story-Part II


Social relationships are a part of all primate groupings.  As means of understanding how these social roles are expressed in captivity, this observational study will incorporate the Francois’ langur population currently residing at the Memphis Zoo.  Emphasis will be placed on the factors contributing to social status as individuals of the group transcend from infant to juvenile to adult status and take a more active role in the social dynamics of the troop. The Memphis Zoo Francois’ langur population currently consists of 2 adult females, one adult male, and currently 3 juveniles, (two male and one female). The study will continue to primarily concentrate on the interactions of the subadults within the group and towards each other. In late spring/early summer of 2009, the group is going to be adjusting to the birth of another infant into the group. This unique social interaction was not present during the previous part of the study and will provide further insights into the group dynamics with the addition of another member. Behavioral observations will emphasize how juveniles of both sexes will participate in the rearing of the infant.

6. A novel approach to chytrid fungus (Batrachochytrium dendrobatidis) detection and prevention in amphibians


Batrachochytrium dendrobatidis (Bd) is a zoosporic fungus that causes chytridiomycosis in amphibians. The rapid global spread of chytridiomycosis has decimated amphibian populations and threatens the Memphis Zoo’s amphibian collection as well. Detection of Bd is labor-intensive and traditionally confirmed histologically or using quantitative polymerase-chain reaction (qPCR) assays to confirm presence of fungal DNA. We propose the development of a rapid, cost effective and noninvasive Near Infrared Reflectance Spectroscopy (NIRS) screening for Bd to prevent widespread infection of amphibians in the Memphis Zoo’s collections and build foundations for Bd detection using the Zoo’s collections to generate discriminative NIRS spectral libraries for healthy verses Bd infected amphibians across multiple species. We will calibrate NIRS spectra against qPCR Bd diagnoses to conduct immediate Bd screening of new additions to the Zoo’s amphibian collection and research colony. We will study 20 amphibian species currently housed at the Herpetarium, as well as and the research colony and local wild populations of Fowler’s toads, Anaxyrus woodhousei fowleri.

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